pco.flim X
PART/ 85108021002

pco.flim X

The pco.flim X is a new version of the first frequency domain (FD) Fluorescence Lifetime Imaging Microscopy (FLIM) camera using a fast modulatable CMOS sensor with two readout-taps per pixel. It offers an improved cooling, which achieves -5°C with forced air cooling and -20°C image sensor temperature with liquid cooling. All of this reduces the readout noise to 33 [e-] and extending the usable exposure times to 4 instead of 2s. Therefore, the pco.flim X remains the frequency domain FLIM camera system with the largest resolution of 1008 x 1008 pixel and the highest maximum frame rate of 45 double image/s on the market.

  • Excellent resolution of 1008 x 1008 pixels
  • Can measure fluorescence lifetimes from 0.5 ns – 100 µs
  • Image sensor thermoelectrically cooled down to -5°C (air) / -20 °C (liquid)
  • Maximum frame rate of 45 double images/s at full resolution

Length 109.0 mm
Weight (kg) 2.4
Dynamic Range (log) 60.0 dB
Forced Air true
Readout Noise RMS 15.0 e-
Pixel Size (H x V) 5.6 x 5.6
Thermo Electric Cooling true
Frame rate (Full Sensor) 45.0 fps
Peak Quantum Efficiency 39.0 %
Peak Quantum Efficiency Wavelength 550.0 nm
Sensor Technology Modulatable CMOS
Sensor Version Custom Design QM-FLIM II
Operational Modes Standard
Dynamic Range A/D Converter 14 bit
Dynamic Range 1024:1
Resolution (H x V) 1008 x 1008
Sensor Diagonal 8.1 mm
Liquid Cooling true
Included Lens Mount C-Mount
Data Interface USB 3.0
Line Scanning false
Global Reset true
Trigger Input Signals exposure start (phase sequence trigger)
Global Shutter false
Region of Interest Vertical (ROI) 1 rows
Non Linearity 1.0 %
MTF (Theoretical) 108.6 lp/mm
Optional Lens Mount F-Mount
TFL-Mount
Spectral Range Minimum 400 nm
Dark Signal Non-Uniformity DSNU 56.0 e- (rms)
Default Read Out Mode Standard
Operating Temperature Range +10 °C to +40 °C
Sensor Manufacturer PCO
Power Supply 90-260 VAC (24 VDC opt.)
Sensor Dimensions (H x V) 5.7 x 5.7
Spectral Range Maximum 950 nm
Direct Mounting Distance (No Camera Flange) 7.4 mm (±10 %)
Chroma mono
Rolling Shutter false
Humidity Range (Non-Condensing) 10 % to 80 % (recommended < 65 %)
Trigger Cable Connector SMA
Double Shutter false
Power Consumption max. 50 W
Storage Temperature Range -10 °C to +60 °C
Compliance Label CE
FCC
Region of Interest Horizontal (ROI) 16 columns
Trigger Output Signals exposure
busy
gate (light enable)
Modulation Frequency External 500 kHz - 40 MHz
Modulation Signal Shape sinusoidal / rectangular
Modulation Frequency Internal 5 kHz - 40 MHz
Data Source v1.0.0
Part Number 85108021002
  • Lifetime from 0.5 ns to 100 µs

  • Frequency synthesizer 5 kHz - 40 MHz
  • Improved cooling of image sensor down to - 5 °C (air) / - 20 °C (liquid)

  • High frame rate 45 double frames / s
  • Unique resolution of 1008 x 1008 pixels
  • Lightsheet Fluorescence Microscopy with Fluorescence Lifetime Imaging
  • High-resolution fluorescence lifetime imaging in widefield microscopy
  • Instant FRET measurements by using the donor fluorescence lifetime
  • 2D measurements with optical chemical sensors
  • Digital staining by using the endogenous fluorescence lifetime
  • Semiconductor quality control
  • Whole Slide Scanning
  • Confocal Spinning Disk Microscopy
  • Solar cell research and quality control (perovskite)
  • Environmental micro plastic detection
  • Improved classification of used wood
  • Use of optical chemical sensors in micro-fluidics

pco.flim X QE Curve

PCO_QE_Curve_flim.X
PCO_QE_Curve_flim.X
Length 109.0 mm
Weight (kg) 2.4
Dynamic Range (log) 60.0 dB
Forced Air true
Readout Noise RMS 15.0 e-
Pixel Size (H x V) 5.6 x 5.6
Thermo Electric Cooling true
Frame rate (Full Sensor) 45.0 fps
Peak Quantum Efficiency 39.0 %
Peak Quantum Efficiency Wavelength 550.0 nm
Sensor Technology Modulatable CMOS
Sensor Version Custom Design QM-FLIM II
Operational Modes Standard
Dynamic Range A/D Converter 14 bit
Dynamic Range 1024:1
Resolution (H x V) 1008 x 1008
Sensor Diagonal 8.1 mm
Liquid Cooling true
Included Lens Mount C-Mount
Data Interface USB 3.0
Line Scanning false
Global Reset true
Trigger Input Signals exposure start (phase sequence trigger)
Global Shutter false
Region of Interest Vertical (ROI) 1 rows
Non Linearity 1.0 %
MTF (Theoretical) 108.6 lp/mm
Optional Lens Mount F-Mount
TFL-Mount
Spectral Range Minimum 400 nm
Dark Signal Non-Uniformity DSNU 56.0 e- (rms)
Default Read Out Mode Standard
Operating Temperature Range +10 °C to +40 °C
Sensor Manufacturer PCO
Power Supply 90-260 VAC (24 VDC opt.)
Sensor Dimensions (H x V) 5.7 x 5.7
Spectral Range Maximum 950 nm
Direct Mounting Distance (No Camera Flange) 7.4 mm (±10 %)
Chroma mono
Rolling Shutter false
Humidity Range (Non-Condensing) 10 % to 80 % (recommended < 65 %)
Trigger Cable Connector SMA
Double Shutter false
Power Consumption max. 50 W
Storage Temperature Range -10 °C to +60 °C
Compliance Label CE
FCC
Region of Interest Horizontal (ROI) 16 columns
Trigger Output Signals exposure
busy
gate (light enable)
Modulation Frequency External 500 kHz - 40 MHz
Modulation Signal Shape sinusoidal / rectangular
Modulation Frequency Internal 5 kHz - 40 MHz
Data Source v1.0.0
Part Number 85108021002
  • Lifetime from 0.5 ns to 100 µs

  • Frequency synthesizer 5 kHz - 40 MHz
  • Improved cooling of image sensor down to - 5 °C (air) / - 20 °C (liquid)

  • High frame rate 45 double frames / s
  • Unique resolution of 1008 x 1008 pixels
  • Lightsheet Fluorescence Microscopy with Fluorescence Lifetime Imaging
  • High-resolution fluorescence lifetime imaging in widefield microscopy
  • Instant FRET measurements by using the donor fluorescence lifetime
  • 2D measurements with optical chemical sensors
  • Digital staining by using the endogenous fluorescence lifetime
  • Semiconductor quality control
  • Whole Slide Scanning
  • Confocal Spinning Disk Microscopy
  • Solar cell research and quality control (perovskite)
  • Environmental micro plastic detection
  • Improved classification of used wood
  • Use of optical chemical sensors in micro-fluidics

pco.flim X QE Curve

PCO_QE_Curve_flim.X
PCO_QE_Curve_flim.X
Close

Subscribe Now!

Join the PCO Circular newsletter for early access to insights on our high‑performance imaging cameras. Receive the latest application notes, expert webinars, technical articles, educational videos, product innovations, and industry events designed for scientific, microscopy, research, and industrial imaging applications.

Floating Web Forms block

Excelitas
Subscribe Now!

Research Article: Live-Cell FLIM Reveals Oxygen Equilibration in Cultured Cells

Click below to download your free copy of the file.

Article: Live-Cell FLIM Reveals Oxygen Equilibration in Cultured Cells

Video: Frequency Domain Fluorescence Lifetime Imaging Microscopy - A Hands-On Session

NOTE: If you have disabled our marketing cookies, you will not be able to access our videos or downloads. To update your cookie preferences, please click here to review and enable cookies.

Video not available due to access restriction to YouTube videos.

Video: Fluorescence Lifetime Imaging FLIM with the frequency domain pco flim system

NOTE: If you have disabled our marketing cookies, you will not be able to access our videos or downloads. To update your cookie preferences, please click here to review and enable cookies.

Video not available due to access restriction to YouTube videos.