pco.flim X
The pco.flim X is a new version of the first frequency domain (FD) Fluorescence Lifetime Imaging Microscopy (FLIM) camera using a fast modulatable CMOS sensor with two readout-taps per pixel. It offers an improved cooling, which achieves -5°C with forced air cooling and -20°C image sensor temperature with liquid cooling. All of this reduces the readout noise to 33 [e-] and extending the usable exposure times to 4 instead of 2s. Therefore, the pco.flim X remains the frequency domain FLIM camera system with the largest resolution of 1008 x 1008 pixel and the highest maximum frame rate of 45 double image/s on the market.
- Excellent resolution of 1008 x 1008 pixels
- Can measure fluorescence lifetimes from 0.5 ns – 100 µs
- Image sensor thermoelectrically cooled down to -5°C (air) / -20 °C (liquid)
- Maximum frame rate of 45 double images/s at full resolution
| Length | 109.0 mm |
| Weight (kg) | 2.4 |
| Dynamic Range (log) | 60.0 dB |
| Forced Air | true |
| Readout Noise RMS | 15.0 e- |
| Pixel Size (H x V) | 5.6 x 5.6 |
| Thermo Electric Cooling | true |
| Frame rate (Full Sensor) | 45.0 fps |
| Peak Quantum Efficiency | 39.0 % |
| Peak Quantum Efficiency Wavelength | 550.0 nm |
| Sensor Technology | Modulatable CMOS |
| Sensor Version | Custom Design QM-FLIM II |
| Operational Modes | Standard |
| Dynamic Range A/D Converter | 14 bit |
| Dynamic Range | 1024:1 |
| Resolution (H x V) | 1008 x 1008 |
| Sensor Diagonal | 8.1 mm |
| Liquid Cooling | true |
| Included Lens Mount | C-Mount |
| Data Interface | USB 3.0 |
| Line Scanning | false |
| Global Reset | true |
| Trigger Input Signals | exposure start (phase sequence trigger) |
| Global Shutter | false |
| Region of Interest Vertical (ROI) | 1 rows |
| Non Linearity | 1.0 % |
| MTF (Theoretical) | 108.6 lp/mm |
| Optional Lens Mount |
F-Mount TFL-Mount |
| Spectral Range Minimum | 400 nm |
| Dark Signal Non-Uniformity DSNU | 56.0 e- (rms) |
| Default Read Out Mode | Standard |
| Operating Temperature Range | +10 °C to +40 °C |
| Sensor Manufacturer | PCO |
| Power Supply | 90-260 VAC (24 VDC opt.) |
| Sensor Dimensions (H x V) | 5.7 x 5.7 |
| Spectral Range Maximum | 950 nm |
| Direct Mounting Distance (No Camera Flange) | 7.4 mm (±10 %) |
| Chroma | mono |
| Rolling Shutter | false |
| Humidity Range (Non-Condensing) | 10 % to 80 % (recommended < 65 %) |
| Trigger Cable Connector | SMA |
| Double Shutter | false |
| Power Consumption | max. 50 W |
| Storage Temperature Range | -10 °C to +60 °C |
| Compliance Label |
CE FCC |
| Region of Interest Horizontal (ROI) | 16 columns |
| Trigger Output Signals |
exposure busy gate (light enable) |
| Modulation Frequency External | 500 kHz - 40 MHz |
| Modulation Signal Shape | sinusoidal / rectangular |
| Modulation Frequency Internal | 5 kHz - 40 MHz |
| Data Source | v1.0.0 |
| Part Number | 85108021002 |
Lifetime from 0.5 ns to 100 µs
- Frequency synthesizer 5 kHz - 40 MHz
Improved cooling of image sensor down to - 5 °C (air) / - 20 °C (liquid)
- High frame rate 45 double frames / s
- Unique resolution of 1008 x 1008 pixels
- Lightsheet Fluorescence Microscopy with Fluorescence Lifetime Imaging
- High-resolution fluorescence lifetime imaging in widefield microscopy
- Instant FRET measurements by using the donor fluorescence lifetime
- 2D measurements with optical chemical sensors
- Digital staining by using the endogenous fluorescence lifetime
- Semiconductor quality control
- Whole Slide Scanning
- Confocal Spinning Disk Microscopy
- Solar cell research and quality control (perovskite)
- Environmental micro plastic detection
- Improved classification of used wood
- Use of optical chemical sensors in micro-fluidics



| Length | 109.0 mm |
| Weight (kg) | 2.4 |
| Dynamic Range (log) | 60.0 dB |
| Forced Air | true |
| Readout Noise RMS | 15.0 e- |
| Pixel Size (H x V) | 5.6 x 5.6 |
| Thermo Electric Cooling | true |
| Frame rate (Full Sensor) | 45.0 fps |
| Peak Quantum Efficiency | 39.0 % |
| Peak Quantum Efficiency Wavelength | 550.0 nm |
| Sensor Technology | Modulatable CMOS |
| Sensor Version | Custom Design QM-FLIM II |
| Operational Modes | Standard |
| Dynamic Range A/D Converter | 14 bit |
| Dynamic Range | 1024:1 |
| Resolution (H x V) | 1008 x 1008 |
| Sensor Diagonal | 8.1 mm |
| Liquid Cooling | true |
| Included Lens Mount | C-Mount |
| Data Interface | USB 3.0 |
| Line Scanning | false |
| Global Reset | true |
| Trigger Input Signals | exposure start (phase sequence trigger) |
| Global Shutter | false |
| Region of Interest Vertical (ROI) | 1 rows |
| Non Linearity | 1.0 % |
| MTF (Theoretical) | 108.6 lp/mm |
| Optional Lens Mount |
F-Mount TFL-Mount |
| Spectral Range Minimum | 400 nm |
| Dark Signal Non-Uniformity DSNU | 56.0 e- (rms) |
| Default Read Out Mode | Standard |
| Operating Temperature Range | +10 °C to +40 °C |
| Sensor Manufacturer | PCO |
| Power Supply | 90-260 VAC (24 VDC opt.) |
| Sensor Dimensions (H x V) | 5.7 x 5.7 |
| Spectral Range Maximum | 950 nm |
| Direct Mounting Distance (No Camera Flange) | 7.4 mm (±10 %) |
| Chroma | mono |
| Rolling Shutter | false |
| Humidity Range (Non-Condensing) | 10 % to 80 % (recommended < 65 %) |
| Trigger Cable Connector | SMA |
| Double Shutter | false |
| Power Consumption | max. 50 W |
| Storage Temperature Range | -10 °C to +60 °C |
| Compliance Label |
CE FCC |
| Region of Interest Horizontal (ROI) | 16 columns |
| Trigger Output Signals |
exposure busy gate (light enable) |
| Modulation Frequency External | 500 kHz - 40 MHz |
| Modulation Signal Shape | sinusoidal / rectangular |
| Modulation Frequency Internal | 5 kHz - 40 MHz |
| Data Source | v1.0.0 |
| Part Number | 85108021002 |
Lifetime from 0.5 ns to 100 µs
- Frequency synthesizer 5 kHz - 40 MHz
Improved cooling of image sensor down to - 5 °C (air) / - 20 °C (liquid)
- High frame rate 45 double frames / s
- Unique resolution of 1008 x 1008 pixels
- Lightsheet Fluorescence Microscopy with Fluorescence Lifetime Imaging
- High-resolution fluorescence lifetime imaging in widefield microscopy
- Instant FRET measurements by using the donor fluorescence lifetime
- 2D measurements with optical chemical sensors
- Digital staining by using the endogenous fluorescence lifetime
- Semiconductor quality control
- Whole Slide Scanning
- Confocal Spinning Disk Microscopy
- Solar cell research and quality control (perovskite)
- Environmental micro plastic detection
- Improved classification of used wood
- Use of optical chemical sensors in micro-fluidics



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